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Description:
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Flow Cytometry Data Analysis using R, Workshop offered by the Canadian Bioinformatics Workshops, Toronto, Ontario Canada, June 17-18, 2013 With the underlying technology rapidly increasing in complexity, Flow Cytometry (FCM) data analysis is becoming more crucial for biological experiments. This workshop aims to provide participants some familiarity with the open source software environment R as an analysis tool for FCM data as they explore the fundamental concepts of taking their data to diagnosis and discovery. Participants will be able to open their FCS files within R, explore their data using various visualizations, perform simple automated pre-processing tasks, quickly generate quality assurance reports to identify potential technical issues with the data, and automate simple gating strategies. As well, some basic clustering techniques will be explained and demonstrated, and new advanced analysis options presented (e.g., flowType, flowDensity, flowMeans, RchyOptimyx, flowClust3.0, SPADE, flowFP/flowBin, SamSPECTRAL). Workshop participants will come away with a better understanding of bioinformatics approaches to FCM analysis, be able to write their own analysis scripts in R to explore their FCM data, as well automate a gating strategy and record statistics of interest (e.g., proportions of cells in speci fic populations of interest. Faculty: Ryan Brinkman Fee: $500 before May 17, 2013 To Register: www.bioinformatics.ca Contact: Michelle D. Brazas, Ph.D. Manager, Knowledge and Research Exchange Ontario Institute for Cancer Research MaRS Centre, South Tower 101 College Street, Suite 800 Toronto, Ontario, Canada M5G 0A3 Tel: 416-673-8502 Cell: 416-357-6687 FAX: 416-977-7446 Toll-free: 1-866-678-6427 ext. 8502 Email: michelle.brazas@oicr.on.ca www.oicr.on.ca
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