Events CYTO U Webinar - 21 Color Flow Cytometry for Immunophenotyping Human Blood

CYTO U Webinar - 21 Color Flow Cytometry for Immunophenotyping Human Blood

Event location: Online at CYTO U
Event date and time: Mar 27, 2018

Presents a New Webinar Series:

Cytometry Part A Spotlight

Part One

21-Color Flow Cytometry for Immunophenotyping Human Blood

Tuesday, March 27 at 12pm EDT

Presented by:

Karl Staser, MD, PhD

Instructor, Department of Medicine
Division of Dermatology
Washington University School of Medicine
St. Louis, MO

About the Presenter

Dr. Karl Staser graduated with an AB in American History and Literature from Harvard College in 2002. He then enrolled in the Indiana University School of Medicine, where he completed a Howard Hughes Medical Institute “year-out” research fellowship for medical students and subsequently entered the Indiana University Medical Scientist Training Program under the supervision of Dr. Wade Clapp. Dr. Staser then joined the Physician Scientist Training Program at Washington University and completed his internal medicine internship and dermatology residency at Barnes-Jewish Hospital in 2017. He is now researching novel therapies for graft-versus-host disease and cutaneous T cell lymphoma in Dr. John F. DiPersio’s lab at the Siteman Cancer Center at Washington University in St. Louis.

Webinar Summary

Our 21-color flow cytometry panel enables simultaneous quantification of monocytes, basophils, granulocytes, dendritic cells, natural killer cells, B cells, and all well-defined T and T helper cell subsets in the human peripheral blood. This panel captures the major phenotypes described in the NIH Human Immunology Project with additional markers for deep T cell analysis.

We specifically designed this panel for peripheral blood analysis from patients involved in our clinical trials of novel agents for the treatment of graft versus host disease (GVHD) after allogeneic hematopoietic stem cell transplantation (alloHSCT). We have optimized this panel for the analysis of 1x10^6 fresh or previously frozen peripheral blood mononuclear cells (PBMCs).

See the original article in Cytometry Part A at:;jsessionid=8F08EA57B8C64C5BE90D221077F844DA.f03t02

Learning Objectives

Upon completion of the webinar participants will be able to:
  • Describe the process of designing and validating a 21-color flow cytometry panel
  • Demonstrate the application of this particular 21-color flow cytometry panel
  • Discuss troubleshooting and experimental refinement of 21-color flow cytometry

Who Should Attend                                      
Physicians, scientists, and other researchers interested in high-color flow cytometry for the rapid and reproducible immunophenotyping of human blood.

CYTO U Webinar Recordings

A recording of this webinar will be posted online at CYTO U within 24-48 hours after the live event for free viewing by all. Recordings of previously presented live webinars are also available. For more details, visit:

CYTO University

CYTO University is an online educational resource created by ISAC for its members and the wider cytometry community.  In addition to free webinars, CYTO U presents recorded courses and tutorials from the CYTO Conference, as well as interactive online courses on a variety of cytometry topics.  These are available at no cost to ISAC members and for a nominal charge for non-members.  Webinars are free for all.

Our vision is to advance the impact of cytometry in meeting current and emerging challenges in the life, biomedical, and physical sciences.

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