Events CYTO U Webinar - Cytometry Part A Spotlight, Part 2

CYTO U Webinar - Cytometry Part A Spotlight, Part 2

Event location: Online at CYTO U
Event date and time: Jun 7, 2018


 

Cytometry Part A Spotlight:

 

Rapid Comparative Immunophenotyping of Human Mesenchymal Stromal Cells by a Modified Fluorescent Cell Barcoding Flow Cytometric Assay


Thursday, June 7 at 10am Eastern (U.S. & Canada)

Presented by:

Tamara Lekishvili, Ph.D.

Researcher, Molecular and Cell Biology
LGC Group
Middlesex, U.K.



 

About the Presenter


Dr. Lekishvili received her Ph.D. from Tbilisi State University, Georgia (Biochemistry). Later she was awarded with the NATO scholarship and continued her research at NTNU (Norway) in neurobiology. After two dynamic post-doctoral positions at the University of Bath (Prion disease research group) and University of Birmingham (Breast Cancer research group), she joined LGC were she is a certified flow cytometry specialist within the cell and molecular biology team. Her major research focus is developing novel cell-based assays using different flow cytometry applications.


Webinar Summary


Standardisation of flow cytometry methods is required to support robust and reliable quality assessment of cells during bioprocessing. Fluorescent cell barcoding (FCB) overcomes some of the limitations of conventional flow cytometry methods, with improved accuracy and data quality. However, measurement variability across instruments or between analysts remains a challenge that must be addressed.
 

A modified fluorescent cell barcoding method using fixable viability dye eFlour 506 has been developed that reduces the potential for such variation. Proof of principle has been demonstrated for multiplexed immunophentoypic analysis using cultured human mesenchymal stromal cells (hMSCs) exposed to a variety of bioprocessing conditions during cell expansion.

This approach, which can easily be transferred to a variety of cell types, represents a further step towards achieving the rigour and robustness required for quality assessment of bioprocessing of cell therapy products, allowing more subtle variations in marker expression levels to be confidently observed.

See the abstract of original article in Cytometry Part A at: https://onlinelibrary.wiley.com/doi/10.1002/cyto.a.23248


Learning Objectives
 

  • Describe the modified cytosolic fluorescent cell barcoding method
  • Provide the critical technical considarations during the FCB method development
  • Demonstare the rapid hMSC immunophenotyping utilising FCB approach


Who Should Attend
 

Scientists and researchers interested in FCB by flow cytometry for the rapid and reproducible immunophenotyping.




CYTO U Webinar Recordings

A recording of this webinar will be posted online at CYTO U within 24-48 hours after the live event for free viewing by all. Recordings of previously presented live webinars are also available. For more details, visit: www.cytou.org.


CYTO University


CYTO University is an online educational resource created by ISAC for its members and the wider cytometry community.  In addition to free webinars, CYTO U presents recorded courses and tutorials from the CYTO Conference, as well as interactive online courses on a variety of cytometry topics.  These are available at no cost to ISAC members and for a nominal charge for non-members. 

Webinars are free for all.
 


Our vision is to advance the impact of cytometry in meeting current and emerging challenges in the life, biomedical, and physical sciences.
 

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