Directed molecular evolution to design advanced red fluorescent proteins
“Directed molecular evolution to design advanced red fluorescent proteins” F.V. Subach, K.D. Piatkevich and V.V. Verkusha, Nature Methods 8, no. 12, 1019-1026 (2011). A summary of strategies for development of red-shifted fluorescent proteins as well as a discussion of improvements in both the biological and instrumentation aspects of screening techniques.