The Southeast revived its flow cytometry interest group this June with a two day meeting in Athens, Georgia. The unofficially named Southeastern Flow Cytometry Interest Group held a meeting on June 4, 2010 at the Georgia Center for Continuing Education at the University of Georgia in Athens, Georgia. The meeting was preceded the day before with a FloCyte course on Multiparameter Flow Cytometry that was attended by 26 enthusiastic flow users and a Core Manager's Meeting that had 36 attendees learning about QC and QA in a multi-cytometer core, how to write a Shared Instrumentation Grant and the importance of standard operating procedures. The meeting itself was attended by 75 participants and 18 vendors. There were speakers from across the Southeast who spoke on canine mammary cancer, T cell immunity in Chagas disease, flow in the environment and therapeutic antibodies in influenza. In the afternoon, we learned about imaging flow cytometry, multiparametric flow in cellular biochemical processes, single cell genomics and adipose stem cells. With excellent support from our vendors, ISAC and the Georgia Center, it was an informative and fun meeting and we hope to do it again next year - June 23 and June 24, 2011 to be exact - and we hope to see you all there!