9 September 2011. “The use of HaloTag-based technology in flow and laser scanning cytometry analysis of live and fixed cells”, Kovalenko, EI, Ranjbar, S., Jasenosky, LD, Goldfeld, AE, Vorobjev, IA, and Barteneva, NS. BioMedCentral (BMC Research Notes 2011, 4:340)
Use of HT technology for cytometric applications has advantages over its use in microscopy as it allows for the statistical measurement of protein expression levels in individual cells within a heterogeneous cell population in combination with cell cycle analysis. Another advantage is the ability of the HaloTag to withstand long fixation and high concentration of fixative, which can be useful in research of infectious agents like HIV and/or mycobacteria.