A multidisciplinary team from France has come up with a robust approach to detect molecular interactions in a reliable way using flow cytometry.
A multidisciplinary team from France has come up with a robust approach to detect molecular interactions in a reliable way using flow cytometry. This technology could lead to the discovery of new drugs via High-Throughput screening.
Various methodological and conceptual advances are required to chart comprehensive protein interaction maps and understand the complexity of networks. Similarly, much also needs to be done in order to characterize protein modulators. In this regard, the FRET approach represents an alternative to study these interactions in vivo. In addition, when the rapidity and multiparametric properties of flow cytometry are combined with the precision and resolution capabilities of FRET, we can obtain a robust and quick approach to analyzing protein interactions in viable cells.
The research team solidified its concept by measuring the activity of caspase 8. They have been able to measure the efficiency of FRET using various fusion proteins in such a way that any intrinsic artifacts related to the capture of fluorescent signals are easily corrected by the software. Up until today this correction was not readily available with classical flow cytometry techniques.
FRETinFLOW’s software, with its unique algorithm, is capable of calculating and counteracting artifacts related to the flow Cytometry in a cell by cell system. Not only that, the software can improve results by only taking into consideration viable cells with fluorescence in the process.
Anabelle Sequeira-Le Grand, PhD.
Université de Bourgogne.
PôleSanté, Faculté de Médecine,
Resp. Plateforme de Cytometrie
7, Bd Jeanne d'Arc 21000 Dijon