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News > Latest Cytometry News > NEW TECHNOLOGY
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NEW TECHNOLOGY


"Clickable, stable fluorophores", Yang, SK et.al., J. Am. Chem. Soc. 133, 9964-9967 (2011). Perylenediimides (PDI's) are stable fluorophores with high quantum yields. Yang et al. report encapsulating PDIs in polyglycerol dendrons along with a single azide group as a ‘click' chemistry handle. These compounds were used to label proteins on the surface of live bacterial and mammalian cells.

"A photoswitchable orange-to-far-red fluorescent protein, PSmOrange", Subach, OM, et al, Nature Methods 8, 771-777 (2011). Monomeric Orange (PSmOrange) protein is photoswitchable. Initially it is orange (excitation, 548 nm; emission, 565 nm) but becomes far-red (excitation, 636 nm; emission, 662 nm) after irradiation with blue-green light.
 

"Beyond the rainbow: new fluorescent proteins brighten the infrared scene", Zin, MZ, Nature Methods 8, 726-728 (2011). A review article describes two fluorescent proteins (PSmOrange and iRFP (infrared fluorescent protein)) that emit in the far-red and infrared range for imaging applications in cells and in vivo. See: Filnov, GS, et al. Nat. Biotechnol. 29, 757-761 (2011) for information about iRFP.



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