New from BioTechniques
Bethune MT, Comin-Anduix B, Fu Y-H H, Ribas A, and Baltimore D., BioTechniques, Vol. 62, No. 3, March 2017; 123–130
Peptide–major histocompatibility complex (pMHC) multimers enable the detection, characterization, and isolation of antigen-specific T-cell subsets at the single-cell level via flow cytometry and fluorescence microscopy. These labeling reagents exploit a multivalent scaffold to increase the avidity of individually weak T-cell receptor (TCR)-pMHC interactions.