Download Your New Proliferation Module with Kylie Price, Kathy Muirhead, and Paul Wallace
April 10, 2018
Proliferation Monitoring by Flow Cytometry
Lesson 2: Monitoring Cell Cycle Progression
Module C: Practical Considerations & Troubleshooting
This is the third of four modules in Lesson 2 of the Proliferation Course, which focuses on cell cycle analysis by flow cytometry. Module 2C: “Practical Considerations and Troubleshooting" examines how choices made during sample preparation, reagent selection, instrument set-up, and data collection impact the reliability and quality of the resulting DNA histograms.
Topics covered include instrument linearity, fixation effects, doublet discrimination techniques, and special considerations for DNA cell cycle analysis using vital DNA dyes and thymidine analogs.
Lesson 1: Basics of the Cell Cycle is currently available here.
Lesson 2A: Principles and Methods is currently available here.
Lesson 2B: DNA Cell Cycle Data Analysis Strategies is currently available here.
Kylie M. Price
Hugh Green Cytometry Core Facility
Malaghan Institute of Medical Research
Wellington, New Zealand
Katharine A. (Kathy) Muirhead
Middleton, Wisconsin, USA
Paul K. Wallace
Department of Flow and Image Cytometry
Roswell Park Comprehensive Cancer Center
Buffalo, New York, USA
CYTO University is an online educational resource created by ISAC for its members and the wider cytometry community. In addition to free webinars, CYTO U presents recorded courses and tutorials from the CYTO Conference, as well as interactive online courses on a variety of cytometry topics. These are available at no cost to ISAC members and for a nominal charge for non-members. Webinars are free for all.