News Download Your New Proliferation Module with Kylie Price, Kathy Muirhead, and Paul Wallace

Download Your New Proliferation Module with Kylie Price, Kathy Muirhead, and Paul Wallace

April 10, 2018



Proliferation Monitoring by Flow Cytometry


Lesson 2: Monitoring Cell Cycle Progression

Module C: Practical Considerations & Troubleshooting

This is the third of four modules in Lesson 2 of the Proliferation Course, which focuses on cell cycle analysis by flow cytometry. Module 2C: “Practical Considerations and Troubleshooting" examines how choices made during sample preparation, reagent selection, instrument set-up, and data collection impact the reliability and quality of the resulting DNA histograms.

Topics covered include instrument linearity, fixation effects, doublet discrimination techniques, and special considerations for DNA cell cycle analysis using vital DNA dyes and thymidine analogs.


Lesson 1: Basics of the Cell Cycle is currently available here.

Lesson 2A: Principles and Methods is currently available here.

Lesson 2B: DNA Cell Cycle Data Analysis Strategies is currently available here.


Kylie M. Price

Hugh Green Cytometry Core Facility
Malaghan Institute of Medical Research
Wellington, New Zealand

Katharine A. (Kathy) Muirhead

SciGro, Inc.
Midwest Office
Middleton, Wisconsin, USA

Paul K. Wallace

Department of Flow and Image Cytometry
Roswell Park Comprehensive Cancer Center
Buffalo, New York, USA


CYTO University

CYTO University is an online educational resource created by ISAC for its members and the wider cytometry community. In addition to free webinars, CYTO U presents recorded courses and tutorials from the CYTO Conference, as well as interactive online courses on a variety of cytometry topics. These are available at no cost to ISAC members and for a nominal charge for non-members. Webinars are free for all.


Our vision is to advance the impact of cytometry in meeting current and emerging challenges in the life, biomedical, and physical sciences.

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