Read the Special Issue on “Stromal Cells in Health and Disease” in the Latest Cytometry Part A
October 22, 2018
Guest Editors: Frank A. Schildberg and Vera S. Donnenberg
Special Issue on “Stromal Cells in Health and Disease” in Cytometry Part A: Journal of Quantitative Cell Science
Read the editorial here.
Here are some highlights:
Evaluation of bone marrow microenvironment could change how myelodysplastic syndromes are diagnosed and treated
. See Mariana and Campos Catafal
Myelodysplastic syndromes are a heterogeneous group of clonal hematopoietic disorders. However, the therapies used against the hematopoietic stem cells clones have limited efficacy; they slow the evolution toward acute myeloid leukemia rather than stop clonal evolution and eradicate the disease. The progress made in recent years regarding the role of the bone marrow microenvironment in disease evolution may contribute to progress in this area. This review presents the recent updates on the role of the bone marrow microenvironment in myelodysplastic syndromes pathogenesis and tries to find answers regarding how this information could improve myelodysplastic syndromes diagnosis and therapy.
Multiplexed fluorescence microscopy reveals heterogeneity among stromal cells in mouse bone marrow sections
. See Holzwarth et al
The bone marrow consists of multiple niches homing hematopoietic cells and stromal cells that fulfill various essential functions such as homeostasis, differentiation, or immunological memory but their composition remains unclear. Here, the authors apply multiplexed immunofluorescence histology data with various object‐based segmentation approaches to define irregularly shaped, net‐like structures of stromal cells and demonstrate that the approach is suitable for spatial analysis of complex tissue structures.
Quantification of airway fibrosis in asthma by flow cytometry
. See Reichard et al
A flow cytometric method to quantify current collagen biosynthesis by lung myofibroblasts is described. Medium and high collagen‐I expressing CD45‐αSMA+ myofibroblasts were identified in murine lungs. In a house dust mite model of allergic asthma, an increase in collagen‐I content in both medium and high expressers was observed, along with an increase in the total number of myofibroblasts expressing high levels of collagen‐I.
READ ALL ARTICLES INCLUDED IN THIS SPECIAL ISSUE HERE