Username: Password:
   
Forgot Password?  
Past Presidents
Biology Applications
Clinical Applications
Technology Applications
Mission
Strategic Plan
History
What is Cytometry?
Councilors
Constitution and Bylaws
Committees & Taskforces
Member Directory
ISAC Associated Societies
ISAC Awards
ISAC's AIMBE Fellows
ISAC Scholars Program
Meeting Endorsement and Sponsorships
Mailing List Policy
Become a Member
Membership Directory
Latest ISAC News
Latest Cytometry News
Science News Feeds
List a Course or Event on ISAC'S website
Calendar
Certification Resources
Education Contacts
Education Materials
ISAC Workshops
Laboratory Layout
IT Structure
Contaminant Classification
Financial Audit Process
Managing Maintenance Cost
Negotiating Capital Purchase
Funding Capital Equipment
Marketing Core Services
Core Subsidization
Estimated Fee
Budget Preparation
Salary Determination
Infrastructure
Performance
Organizational Items
Education
Publication
Facilities
Fiscal Issues
Organization
Web Links
ICE Format
Flow Cytometry Data File Format Standards
Image Cytometry Data File Format Standards
Instrument calibration
Fluorescence intensity calibrations
Counting standards
Immunophenotyping
Clinical cytometry standards
Background and Organization
Data File Standards
Calibration and Measurement Standards
MIFlowCyt
Biosafety
Training and Education
Core Managers
Standards
Flow Cytometry Data Repository
Positions Available
Purdue List
Microvesicle Analysis Interest Development Group
About ISAC
Join ISAC
Members Only
Renew Your Membership
News
Course & Meetings Calendar
CYTO/Congress
Publications
Education Resources
Resources for Cytometrists
Cytometry Certification
Interest Groups
Contact Us

Hands-On Flow Cytometry Courses, National Centre for Biological Sciences Details

The 18th International Congress of Cytology Details

Stem Cells and Aging Symposium plus Flow Cytometry Course Details

View All Events
News > Latest ISAC News > A Universal Nanoparticle Cell Secretion Capture Assay? Read about it in the February 2013 Cytometry
** When copying from Microsoft Word Documents you must use the word icon on the editor tool

A Universal Nanoparticle Cell Secretion Capture Assay? Read about it in the February 2013 Cytometry Part A


Secreted proteins play an important role in intercellular interactions. As outlined in their article, "A Universal Nanoparticle Cell Secretion Capture Assay," Wendy Fitzgerald and Jean-Charles Grivel have developed a universal assay that allows a simple non-invasive identification and isolation of cells based on their secretion of various products.

Their method is based on the targeting, to the cell surface, of heterofunctional nonoparticles coupled to a cell-surface specific antibody and to a secreted-protein-specific antibody, which captures the secreted protein on the surface of the secreting cell.  This method allows virtually limitless flexibility and furthermore does not compromise cell viability allowing culture and expansion of the secreting cells

This article can be found in the February 2013 Cytometry Part A journal in pages 205 - 211, or online at http://onlinelibrary.wiley.com/doi/10.1002/cyto.a.22167/full

ISAC members receive the journal Cytometry Part A as a benefit of their ISAC membership.  If you would like to become a member of ISAC, you can join online.

 

Return to home