Winfried Wiegraebe, PhD
Director, Microscopy and Image Analysis
Allen Institute for Cell Science
Seattle, WA, USA
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About the Presenter
Winfried Wiegraebe, PhD
In summer 2015, Winfried Wiegraebe joined the Allen Institute for Cell Science in Seattle, Washington, where he develops and oversees the Institute’s microscopy and image processing pipeline as Director of Microscopy. He came from the Stowers Institute for Medical Research in Kansas City, Missouri, where he created the group for Advanced Instrumentation and Physics and later became the Head of the Stowers Microscopy Center. After a short post-doc, he worked at the optical company Carl Zeiss in R&D, product management, and sales support. Dr. Wiegraebe received his diploma in Biophysics and Machine Tools and Industrial Management from the Technical University in Munich, Germany. For his diploma and Ph.D. thesis, he joined the Department of Molecular Structural Biology headed by Wolfgang Baumeister at the Max-Planck Institute for Biochemistry in Martinsried, Germany. There he developed and applied Scanning Tunneling (STM), Atomic Force (AFM), and Scanning Near Field Optical (SNOM) microscopes to address pressing biological questions.
At the Allen Institute for Cell Science, we believe that understanding the organization of healthy, living cells and their changes during growth, differentiation and other processes is an essential starting point to understand cellular changes caused by disease. Now, the textbook cartoons of the human cells that we are familiar with are based more on imagination than on solid data. To develop the needed image-based cellular data, we are developing a pipeline to create large, high replicate data sets for us and other scientists in academia and industry to analyze, model, and generate new hypothesis about cellular behaviors. We use gene-edited hiPS cells, since they are diploid, relatively homogeneous, and can be induced to differentiate into many other cell types. In the first iteration, we are identifying the locations of the major cellular machines and signaling pathways using genome-edited fluorescently tagged proteins that we image with automated light microscopes. We are analyzing these image-data using statistical models.
The Institute is committed to open science. We will not only share all our data, tools, and other results with the scientific community but also will have an ongoing dialog on tools and questions to address. We depend on your input and feedback to build a pipeline that best complements the achievements and needs of the scientific community.
After the webinar, attendees will have a better understanding about the data and tools the Allen Institute for Cell Science produces and how to obtain and use them. We will discuss the principals and challenges of building a pipeline that combines gene editing, automated microscopy and image processing, and modeling.
Who Should Attend
This webinar will present useful information for people interested in light microscopy and automation. Cell biologists will learn about new hiPS cell lines that might be useful for them. Scientists interested in modeling of cell processes will hear about data sets we are producing and plan to share in the future.
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