Read about Fenton chemistry for BrdU determination in the latest Cytometry Part A!
The use of copper ions and ascorbic acid has been previously described for the detection of cells that incorporate EdU through a “click chemistry” approach (copper-catalyzed cycloaddition). Cappella, Giansanti, Pulici, and Gasparri demostrate that, alongside “click” cycloaddiction, Fenton reactions may also occur under particular experimental conditions, catalyzing the formation of reactive hydroxyl radicals which introduce DNA double-strand breaks and allow immunodetection of incorporated halogen deoxyuridine in viable cells. Fenton and “click” reactions can be combined for BrdU/EdU pulse-and-case experiments and can be employed for multiplexed analysis of DNA replication and immunofluorescence of labile epitopes.
From “Click” to “Fenton” chemistry for 5-bromo-2′-deoxyuridine determination
Paolo Cappella, Vincenzo Giansanti, Maurizio Pulici and Fabio Gasparri
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