Past ISAC Scholar Silas J. Leavesley Assesses FRET Using Spectral Techniques in the Latest Cytometry Part A
Past ISAC Scholar Silas J. Leavesley and coworkers provide a detailed study of Förster resonance energy transfer (FRET) methods, techniques that are invaluable in the probing of phenomena at the single-cell level or assessing intracellular protein–protein interactions. In the authors’ own words, “The purpose of this study is to compare the effectiveness of traditional fluorescence filter set methods to spectroscopic and spectral imaging methods for estimating FRET response.”
Assessing FRET using spectral techniques
Silas J. Leavesley, Andrea L. Britain, Lauren K. Cichon, Viacheslav O. Nikolaev and Thomas C. Rich
The accompanying Commentary by Herbert Schneckenburger not only frames the article in a historical perspective but also underscores the value of this study.
Commentary: Assessing FRET using spectral techniques