Programs Marylou Ingram Scholars

ISAC Marylou Ingram Scholars Program

The ISAC Marylou Ingram Scholars Program (IMISP) is a five-year career development program designed to enhance the scientific and leadership experiences of research leaders in the field of cytometry.  ISAC is seeking candidates specializing in state-of-the-art biomedicine, technology, and other areas of emerging sciences relevant to cytometry. 

The IMISP is foremost a community of approximately 25 cytometrists at similar stages in their careers. It provides leadership training, mentorship with another ISAC member, presentation opportunities, a complimentary membership in the Society, a subscription to Cytometry Part A, complimentary registration and partial travel funds to attend the annual CYTO conferences, eligibility for collaboration/training funding, and other valuable professional development activities.

ISAC Marylou Ingram Scholars are expected to be an integral part of the Society through committee memberships, advisory boards and various taskforces, reviewing manuscripts submitted to Cytometry Part A, and creating educational material to further support the goals of ISAC. Being chosen as an ISAC Marylou Ingram Scholar is an indication that you are recognized for your scientific skills, research accomplishments, leadership potential and ability to achieve your career goals.

Individuals who have received their relevant doctoral degree within six years of the date of the application are eligible to apply.  These requirements may be waived in exceptional circumstances to be evaluated on an individual basis.  Awards of ISAC Marylou Ingram Scholars are highly competitive and awardees are chosen by the ISAC Marylou Ingram Scholars Program Committee (IMISPC). Unsuccessful applicants are encouraged to reapply for the next year provided they continue to meet eligibility criteria.  

The application period for the 2019-2023 term is now closed.

Leaders or prospective leaders in the field of cytometry whose role is primarily operating a shared resource laboratory or educational program should instead apply to the Shared Resource Laboratory (SRL) Emerging Leaders Program. The IMISPC reserves the right to forward applications to the SRL EL Program.

For more information, email Matt Niner at

Current ISAC Marylou Ingram Scholars


Term: 2018-2022

Dominic Jenner
Senior Scientist
Defence Science & Technology Laboratory
Salisbury, United Kingdom
B.S.c (Hons) Microbiology & Zoology, University of Wales, Aberystwyth 1999-2002
Ph.D. Subunit Vaccine development, Open University 2005-2009
Scientific Interest and Avenues for Collaboration
My area of research is focused mainly on using flow cytometry, imaging flow cytometry and image cytometry to evaluate three broad areas: immunology of infectious disease, evaluating the cellular trafficking of toxins and the mechanism of protection of toxin therapeutics, and the immune response to trauma and methods to increase cellular health to traumatic injury.
Participation/Support of ISAC
2015: Cyto2015 Glasgow:
Poster: Using Multispectral imaging flow cytometry to assess ricin trafficking in human lung epithelial-like A549 cells
Talk at Mucosal Immunology workshop: Using Multispectral Imaging Flow Cytometry to assess ricin trafficking in human lung epithelial-like A549 cells
Cyto2016, Seattle: Workshop organizer - Imaging Flow Cytometry: Applications and Challenges
Cyto2017, Boston: Poster - Measuring opsonisation of Burkholderia thailandensis using imaging flow cytometry
Cyto2018, Prague - Workshop organiser: A successful imaging flow cytometry facility: Guidelines for management and publication

Orla Maguire
Senior Flow Cytometry Specialist
Dept. of Flow and Image Cytometry
Roswell Park Cancer Institute
Buffalo, NY, USA
Post-doctoral fellow, Roswell Park Cancer Institute, Buffalo, NY
Ph.D. Biomedical Sciences, Ulster University, Northern Ireland, UK, 2008
B.Sc. (HONS) Biomedical Sciences, Ulster University, Northern Ireland, UK, 2004
Scientific Interest and Avenues for Collaboration
My research interests are focused on developing applications of imaging flow cytometry (IFC), with special attention to cell signaling. My recent focus has been in the effect of chemotherapies on transcription factor pathways in immunophenotypically-defined T cell populations. I am also interested in measuring cellular communication via exosome signaling using IFC.
Participation / Support of ISAC
ISAC member since 2011
Reviewer for Cytometry Part A since 2017
CYTO Program committee member since 2015
CYTO 2011 Poster: Time kinetic study of p65 phosphorylation events in relation to
NF-κB nuclear translocation
CYTO 2013 Oral presentation: Image Cytometry-Based Detection of Aneuploidy by FISH-IS
CYTO 2013 Poster: Simultaneous assessment of CMV specificity and functional response in CD8+ T cells from bone marrow transplant recipients
CYTO 2014 Poster: NF-κB, NFAT1 and ERK1/2 signaling kinetics following allogeneic hematopoietic cell transplantation
CYTO 2016 Poster: Ovarian carcinoma ascites-derived extracellular vesicles inhibit NFAT signaling in antigen-specific T-cells
CYTO 2017 Oral presentation: MHC multimers induce a functionally relevant activation of the NFAT pathway

Peng Qiu
Associate Professor                                      
Georgia Institute of Technology and Emory University
Atlanta, GA, USA                                  
1999-2003, B.S. in Electrical Engineering, University of Science and Technology of China, Hefei, China
2003-2007, Ph.D. in Electrical and Computer Engineering, University of Maryland, College Park, MD, USA
2007-2010, Postdoctoral Researcher at Center for Cancer Systems Biology, Stanford University, CA, USA
Scientific Interest and Avenues for Collaboration
Machine learning and visualization methods for single-cell data, ranging from flow cytometry, CyTOF, single-cell RNAseq, and image-based profiling.
Image analysis for cell tracking and dynamic modelling in microfluidic applications.
Differential equation modeling for experimental design and model reduction.
Integrative analysis of multi-omics data and clinical data for biomarker identification.
Participation / Support of ISAC
Reviewer for Cytometry Part A, 2011-present
Participant in FlowCAP-II, -III and -IV challenges, 2011-2014
CYTO attendee, 2013-2015, 2017-present
Invited speaker for CYTO 2013 pre-congress course on advanced data analysis
Winner of CYTO 2017 Image Analysis Challenge

Aaron Tyznik
Staff Scientist             
BD Biosciences
San Diego, CA, USA
Ph.D. (Immunology), University of Washington School of Medicine, Seattle, WA
Postdoctoral Fellow, La Jolla Institute for Allergy and Immunology, La Jolla, CA
Scientific Interest and Avenues for Collaboration
A cellular immunologist by training, I have a strong interest in engineering and enabling easy adoption and application of flow cytometry. This includes advancing flow cytometry through education to minimize the challenges faced by researchers, and also drive for advances in the technology to reduce the need for this education. These advances include instrument setup, standardization, design, and addressing the challenges of utilizing fluorescent particles in the development of multi-parameter polychromatic panels.
Participation / Support of ISAC
CYTO attendee and presenter (2014-present)

Sofie Van Gassen
Data Mining and Modeling for Biomedicine Group
VIB Inflammation Research Center & Ghent University
BSc Computer Science, Ghent University, 2011
MSc Mathematical Computer Science, Ghent University, 2013
PhD Computer Science Engineering, Ghent University, 2017
Scientific Interest and Avenues for Collaboration
My main research interest is the design of computational solutions for single-cell data analysis. During my PhD, I developed the FlowSOM and FloReMi algorithms for flow cytometry visualization and biomarker discovery, and I am still improving them further. Currently, I am also developing machine learning algorithms for mass cytometry and imaging cytometry datasets. I am especially interested in collaborations with wet lab scientists who are exploring questions to which the current computational tools do not provide the answers yet and who want to explore new machine learning approaches.
Participation/Support of ISAC
CYTO 2017 Oral presentation
CYTO 2016 Poster presentation
CYTO 2015 Poster presentation
CYTO 2014 Poster presentation

Term: 2017-2021

Thomas Myles Ashhurst

High-Dimensional Cytometry Specialist
Sydney Cytometry Core Facility and Discipline of Pathology
The University of Sydney
B.Sc. (Hons): Immunology and Virology, 2011
Finalizing Ph.D. in Medicine: Pathology, pending completion 2017
Scientific Interest and Avenues for Collaboration
My research is focused on analysing cellular infiltration in the virally infected CNS, and understanding how the haematopoietic system is reorganised in the bone marrow to change cellular output in response to infection. To do this, we utilise high-dimensional cytometry systems (such as mass cytometry and 29-parameter next generation fluorescence cytometry), to interrogate cellular systems in a range of disease contexts. To aid our analysis, we apply and improve existing computational analysis tools, in addition to developing our own novel algorithms for analysing high dimensional datasets. All of this work is performed in a team context, and we are always eager for new collaborations on
multiple fronts.
Participation / Support of ISAC
Member of ISAC, and CYTO attendee since 2013
Member of partner organisation, the Australasian Cytometry Society (ACS) since 2012
Outstanding poster presentation award (CYTO 2013)
Student travel awards (multiple)
ACS student liaison (2014 - 2016)
ACS Polychromatic Roadshow co-instructor (2017): Wellington (New Zealand), Perth, Brisbane, Sydney, Melbourne (Australia)
Invited speaker (CYTO Asia, October 2017)

Kanutte Huse

Dept. of Cancer Immunology
Head Engineer
Dept. of Core Facilities
Institute for Cancer Research
Oslo University Hospital

M.S. in Biophysics, University of Oslo, 2005.
Ph.D in Cancer Immunology, Institute for Cancer Research, Oslo University Hospital, 2011.
Scientific Interest and Avenues for Collaboration
I specialize in mass cytometry and phospho-flow, and have an interest in analysis of high-dimensional cytometry data. My main field of interest is B-cell lymphoma and immune responses in cancer, with a focus on immune checkpoints.
Participation/Support of ISAC   
CYTO attendee 2016

Florian Mair

Postdoctoral Fellow   
Fred Hutchinson Cancer Research Center
Seattle, WA

MSc Molecular Biology (University of Vienna, 2008)
PhD Immunology (University of Zurich, 2013)   
Scientific Interest and Avenues for Collaboration
In the past I have been working with flow and mass cytometry to understand the function of dendritic cells during inflammation and autoimmunity. During my current position at Fred Hutch my main interest is delineating innate immune components relevant in anti-cancer immunity. In addition, I am very interested in technical developments for high-dimensional flow cytometry using classical as well as spectral flow cytometry, and novel analysis approaches for the resulting datasets. Also, I have been actively involved in designing and teaching flow cytometry courses at various levels.
Participation / Support of ISAC
2016: CYTO conference in Seattle   
2011-2016: Organization of flow cytometry courses at UZH
2015: CYTO conference in Glasgow
2012: CYTO conference in Leipzig

Henrik Mei

Scientific Head of Mass Cytometry
German Rheumatism Research Center (DRFZ), a Leibniz Institute

Diploma (Medical Biotechnology), Technical University Berlin, Germany
Ph.D. (Immunology), Humboldt University Berlin, Germany
Postdoctoral Fellow, Human Immune Monitoring Center, Stanford, CA, USA
Scientific Interest and Avenues for Collaboration
I am interested in the homeostasis of antibody-secreting plasmablasts and plasma cells, and how their regulation relates to the stability of protective and harmful antibody titers. I pursue this in the context of therapy of rheumatic diseases, where autoreactive B cell memory represents an important obstacle. My research involves cutting edge flow cytometry, and more recently, novel mass cytometry, for which I have developed new reagents and cell sample multiplexing suitable for immune phenotyping studies. I employ mass cytometry to capture the heterogeneity of cellular entities, and for predictive biomarker/biosignature identification in chronic inflammatory diseases.
Participation/Support of ISAC
CYTO attendee since 2014
Reviewer for Cytometry Part A since 2015

Nao Nitta

Associate Program Manager
Japan Science and Technology Agency
Visiting Researcher
University of Tokyo

B.S. University of Tokyo, 2001
M.S. University of Tokyo, 2003
Ph.D. University of Tokyo, 2006
Scientific Interest and Avenues for Collaboration
Image and Flow Cytometry
Single cell genomics
Commercialization and entrepreneurship
I have 13 years of experience in industry (5 years at a biotech startup and 8 years at Sony) and commercialized some cytometry instruments (flow cytometer, chemotaxis & degranulation assay system, etc.).
Participation / Support of ISAC
CYTO Program comittee: CYTO2017
CYTO Attendee: 2008-present

Term: 2016-2020

Sara De Biasi

Post Doc
Department of Surgery, Medicine, Odontoiatrics and Morphological Sciences
University of Modena and Reggio Emilia

Ph.D., University of Modena and Reggio Emilia, Italy (supervisor: Prof. Andrea Cossarizza)
Post-doctoral fellow, Department of Surgery, Medicine, Odontoiatrics and Morphological Sciences (supervisor: Prof. Andrea Cossarizza)
Scientific Interest and Avenues for Collaboration
I am interested in the dynamics of T cell homeostasis and functions in conditions of acquired immunodeficiencies, with a special focus on HIV infection and solid organ transplantation and related therapies. More recently, my work focused on multiple sclerosis as a model to study autoimmunity. In particular, I am investigating the role of innate-like T cells (iNKT cells), rare cells populations among peripheral blood mononuclear cells. In addition, I took advantage of the experience acquired in the field of rare events detection such as iNKT cells to study circulating endothelial cells (CEC), their precursor (EPC) in different type of cancers.
Participation/Support of ISAC
CYTO 2017: T cells from patients with primary progressive form of Multiple Sclerosis display altered metabolism and mitochondrial functionality; Patients with severe sepsis display low levels of circulating Mucosal Associated Invariant T cells; High CD4:CD8 ratio and length of treatment independently correlate to the HIV-DNA intracellular content in different subsets of CD4+ T cells from virologically suppressed HIV+ patients; LonP1 differently modulates mitochondrial function and bioenergetics of primary versus metastatic colon cancer cells (Poster presentation).
CYTO 2016: High Level of Activation and Altered Mitochondrial Functionality in T Cells from Patients with Progressive Forms of Multiple Sclerosis (Oral presentation).
CYTO 2015: Polyfunctional Response of Invariant Natural Killer T Cells in Patients Affected by Multiple Sclerosis Displays Th-1 and Th-17 Profiles (Outstanding poster Award). Inhibition of Lon protease by triterpenoids alters mitochondria and is associated to cell death in human cancer cells (Poster prensentation). iNKT Cells and Their Subpopulations Are Not Restored in HIV+ Patients with Low CD4/CD8 ratio after Prolonged Effective Therapy, and Display a Pronounced Th-1 and Th-17 Pro-inflammatory Profile (Poster prensentation).
CYTO 2014: Fine tuning of Treg and iNKT cells after treatment with Fingolimod in Multiple Sclerosis patients (Oral presentation). Principal Component Analysis allows to cluster patients with Multiple Sclerosis on the basis of different subsets of CD8+ and iNKT cells (Oral presentation).
CYTO 2013: Complex changes in invariant Natural Killer T (iNKT) cells in patients with different clinical forms and treatments of Multiple Sclerosis (Oral presentation).
CYTO 2012: Evidences for Autophagy in SW872 Adipocytic Cells Treated with Atazanavir (Poster presentation).

Matthew Linden

Associate Professor of Haematology
School of Biomedical Sciences
University of Western Australia
Nedlands, WA

BSc Hons PhD W.Aust
I earned my PhD in haematology from The University of Western Australia in 2003 and joined the Centre for Platelet Function Studies at The University of Massachusetts Medical School (now the Centre for Platelet Research and Harvard Medical School) initially as a postdoctoral fellow and then research instructor.
In 2007 I returned to Australia as an academic at RMIT University in Melbourne, where I launched the RMIT Flow Cytometry core facility in 2009. In 2012 I was recruited to the University of Western Australia as academic head of the cytometry core at the Centre for Microscopy, Charactisation and Analysis (a node of the National Microscopy and Microanalysis Research Facility). In 2015 I took up an associate professorship of haematology at the same institution in order to develop postgraduate haematology coursework and progress research on platelets systems biology.
Scientific Interest and Avenues for Collaboration
I am a highly motivated and well-rounded academic with a passion for cytometry. I hold significant roles spanning teaching, research and leadership of cytometry in Australia. Working at the interface of the shared resource laboratory, discovery and translational research, I have developed novel cytometry techniques for the measurement of blood platelets and employed these in the development of new antiplatelet therapies. I am committed to advancing cytometry through strong, sustainable shared resource laboratories and cutting edge research. Through participation in the ISAC Scholar program, I seek to expand across research, education and leadership of cytometry at an international level.
My research focuses on two main fields; advancing understanding of blood platelets in health and disease (funded by the National Health and Medical Research Council of Australia), and advancement and development of cytometry tools for cell biology (funded by the Australian Research Council Discovery Grants Program).  I have developed widely adopted flow cytometric methods for the laboratory monitoring of antiplatelet therapy, applied these to develop novel approaches to inhibition of platelet function, and extended this research and developed cytometric methods for measuring the interaction of platelets with immune cells. I also develop novel flow, imaging and mass cytometry approaches which broadly underpin research in other areas of biological and medical sciences.
Participation / Support of ISAC
I was chair the 38th Annual Scientific Meeting of the Australasian Cytometry Society (ACS) (the Australian affiliate of ISAC), which I hosted in Perth in 2015. I am treasurer of CYTO ASIA, a joint meeting of the ACS, ISAC and Singaporean Society for Immunology, which will be held in Singapore in October 2017.
I continue to be an active member of ISAC and seek to represent cytometry interests in the rapidly expanding Australasian region. I sit on the membership services committee and continue to expand my involvement in ISAC through the Scholar’s program. In 2017 I have submitted an abstract for consideration at CYTO, characterizing platelet-induced signal transduction in metastatic breast cancer cells using mass cytometry.

Jakub Nedbal

Product Development Lead
Photon Force

King's College London, UK - PhD Molecular Immunology, 2012
King's College London, UK - MSc Immunology, 2007
Masaryk University Brno, Czech Republic - MSc Physics, 2006
Scientific Interest and Avenues for Collaboration
I have past record of development of prototype instrumentation for advanced flow cytometry, with specific focus on measurement of fluorescence lifetime to understand protein-protein interactions and molecular changes inside cells. Currently, I am leading the technology development in a recent spinout specializing in advanced quantum-enhanced image sensors. This emerging technology promises breakthroughs in various scientific and industrial applications. These will happen through direct replacement of existing technologies for single-photon detection with more efficient and less costly alternatives. However, unforeseen applications and opportunities are expected to arise because of the unprecedented performance of these new image sensors.
If you are interested in instrumentation development, time-resolved imaging or flow cytometry, please come and find me at CYTO or drop me a line. I’d love to explore ways to apply our sensors to your applications.
Participation/Support of ISAC
Member of the Finance committee, eLearning Education Delivery Task Force and Image Cytometry Task Force
CYTO 2017 Poster presentation
CYTO 2016 Poster Judge, Oral presentation
CYTO 2015 Oral presentation (President's Award for Excellence)
CYTO 2014 Poster presentation

Yvan Saeys

Associate Professor, Ghent University
Group Leader, Data Mining and Modeling for Biomedicine Group
VIB Inflammation Research Center

I obtained my PhD. in Computer Science from Ghent University. After spending time abroad at the University of the Basque Country and the Université Claude Bernard, I obtained a FWO postdoctoral fellowship at Ghent University. Since 2015 I have been an Associate Professor at the faculty of medicine at Ghent University and a Principal Investigator at the VIB Inflammation Research Center. I am heading the Data Mining and Modeling group, an interdisciplinary research team of 15 people consisting of mathematicians, computer scientists, engineers and bioinformaticians.
Scientific Interest and Avenues for Collaboration
The main research theme of my group is the design and application of data mining and machine learning techniques for high-throughput single-cell technologies.  These include flow and mass cytometry based techniques but also single-cell transcriptomics platforms. Our group developed the FlowSOM and FloReMi R packages for flow cytometry visualization and biomarker discovery, and obtained the best results at the FlowCAP IV challenge on predicting HIV to AIDS progression.  We are actively welcoming collaborations with wet-lab groups that would like to explore computational flow cytometry, as well as experimentalists that design new technologies and willing to explore the potential of machine learning techniques for data analysis and interpretation.
Participation/Support of ISAC
I participated in CYTO 2014, where I enjoyed the data analysis sessions and FlowCAP discussions. 
At CYTO 2016 my group had both an oral and a poster presentation. 
At CYTO 2017 my group has an oral presentation and a poster. 
Member of the ISAC Finance committee.
Within ISAC I am actively promoting computational flow cytometry and assist in training the next generation of scientists to get acquainted with the novel tools that computational flow cytometry offers.

Matthew Saunders

Scientist BioLegend (2017)
Research Scientist
Glycosensors and Diagnostics, LLC (2015-2017)
Athens, GA/San Diego, CA

B.S. Biochemistry, University of Oregon, 2002
Ph.D. Biomedical Science, University of New Mexico, 2009
Graduate research support from Los Alamos National Laboratory, National Flow Cytometry Resource (NFCR)
Postdoctoral Researcher, Carnegie Mellon University, Molecular Biosensor and
Imaging Center, 2010-2014
Scientific Interest and Avenues for Collaboration
Flow cytometry based assay development and optimization; Multiplex flow cytometry; Directed evolution and protein engineering; Glycan-specific detection and measurement; Product development and commercialization; Biomarker and immunoassay development
Participation / Support of ISAC
Oral Presentation CYTO 2008 (Budapest, Hungary)
Oral Presentation CYTO 2010 (Seattle, WA)
Poster Judge CYTO 2016 (Seattle, WA)
CYTO Innovation Planning Committee CYTO 2017 (Boston, MA)

Term: 2015-2019

Greg Finak

Senior Staff Scientist  and Vaccine Immunology Statistical Center Methods Development Lead
Department of Biostatistics, Bioinformatics and Epidemiology
Vaccine and Infectious Disease Division
Fred Hutchinson Cancer Research Center
Seattle, WA

MSc in Biochemistry, 2002, McGill University, Montreal, Canada.
PhD in Bioinformatics, 2008, McGill University, Montreal, Canada.
Post-doctoral researcher at the IRCM, Montreal, Canada 2008-2010 and at the Fred
Hutchinson Cancer Research Center, Seattle, WA, 2010-2012.
Scientific Interest and Avenues for Collaboration
My research is focused on developing tools and statistical methods for analysis of high throughput immunological assay data, focusing on applications to biomarker discovery in immunology, vaccine development, and clinical trials. Novel assays, interesting study designs and data sets that pose analytic, computational and statistical challenges are of interest.

Pia Kvistborg

Group Leader, Department of Immunology Education
Netherlands Cancer Institute
The Netherlands

PhD in Tumor immunology from University of Copenhagen
Scientific Interest and Avenues for Collaboration
The main interest of the Kvistborg lab is to dissect the (dys)functional state of tumor-specific T cells in cancer and how anti-cancer therapies may influence this state using RNA sequencing and high-dimensional flow cytometry. For this purpose we are currently setting up flow panels on the Symphony A5 and testing gene expression profiles for suitable for low T cell numbers. At this point in time we are mainly focused on neo-antigen specific T cells in melanoma, head and neck cancer, ovarian cancer and renal cell cancer, and how T cells from patients with these malignancies respond to mono- or combination-therapy with checkpoint targeting antibodies.

Elisa Nemes

Senior Scientist
South African Tuberculosis Vaccine Initiative
Cape Town
South Africa

MSc in Medical Biotechnology and PhD (topic: Immunological effects of antiretroviral therapy interruption in HIV infection) from University of Modena and Reggio Emilia, Italy.
Scientific Interest and Avenues for Collaboration
Clinical trials of novel tuberculosis vaccines
Prospective immunological biomarkers of risk of tuberculosis disease and
immune reconstitution inflammatory syndrome
Paediatric immunology
Development and standardization of novel assays to measure immune cell function and phenotype
Immunodiagnostics for tuberculosis
Participation / Support of ISAC
Attendance at CYTO 2014 (oral presentation), 2015 (session co-chair), 2016 (abstract review and workshop evaluation, poster presentations), 2017 (abstract review, session co-chair, oral presentations).

Raluca Aura Niesner

Group Leader, Biophysical Analytics
German Arthritis Research Center

PhD thesis in Biophysical Chemistry at TU, Braunschweig (2001–2005)
Diploma in Chemistry at TU Braunschweig (May 2001)
Scientific Interests and Avenue for Collaboration
The development of new techniques which better meet the general requirement in biosciences and biomedicine to monitor vital processes at high spatial and temporal resolution, on a molecular basis, in their genuine environment is of central relevance for biophysics in general, and for microscopy in particular. In this frame, we focus on the improvement and extension of multi-photon laser-scanning microscopy (MPLSM) with application to intravital microscopy (imaging in living rodents) to better understand immunological processes in different disease contexts. Thereby, we focus on the improvement of optical performance, e.g. spatial resolution, imaging depth, photobleaching/ phototoxicity, imaging time window, and of molecular selectivity to monitor cell and tissue functions by time-resolved fluorescence techniques.
Participation/Support of ISAC
Invited talk: CYTO2011, CYTO2014
Talk: CYTO2013, CYTO2017
Chair: CYTO2012, CYTO2014, CYTO2015, CYTO2016
Reviewer: CYTO2012, CYTO2014, CYTO2015, CYTO2016
Organizing committee: CYTO2011, CYTO2014, CYTO2017

Josef Spidlen

Director of Bioinformatics
FlowJo, LLC
Ashland, OR

Postdoctorate Fellow, 2005 - 2008, BC Cancer Agency, BC, Canada
Ph.D. in Biomedical Informatics, 2002-2005, Charles University in Prague, First Faculty of Medicine, Czech Republic
M.Sc. in Computer Science, 1996-2002, Charles University in Prague, Faculty of Mathematics and Physics, Czech Republic
Scientific Interest and Avenues for Collaboration
My scientific interest and research are focused on the development and application of software tools for single cell sequencing and automated high-throughput cytometry data analysis. My formal training is in computer science and software engineering, which I have been applying in the fields of medical informatics and computational biology. I am the author and maintainer of several R packages, and the author of several flow cytometry data analysis tools, including most of the modules in the GenePattern Flow Cytometry Suite, and the FlowClean FlowJo plugin. I am also the main author of most recent ISAC data standards, a.k.a. file formats, such as FCS, Gating-ML, CLR, ICEFormat, and for the past 5 years, I was the chief developer of FlowRepository - the largest open source database of public flow cytometry data. In the Fall of 2016, after 11 years at the BC Cancer Agency, I joined the FlowJo, LLC team to help bring advancements of computational biology to researchers without programming skills. Currently, I am mostly focused on creating tools and algorithms for tertiary single cell sequencing data analysis, and I am designing and implementing novel approaches in our SeqGeq™ application. I am always excited to engage in collaborations, especially when it comes to improving FlowJo® or SeqGeq™ to address data analysis challenges to gain novel biological insights.
Participation / Support of ISAC
ISAC Data Standards Task Force: first author of most ISAC data standards.
FlowRepository Steering Committee: Chief developer of FlowRepository 2012-2016.
Member of the CYTO 2016 Parallel Session Committee.
Attended many past CYTO conferences, taught at workshops, helped by chairing sessions and judging posters.

Term: 2014-2018

Jessica P. Houston

Associate Professor    
Department of Chemical & Materials Engineering
New Mexico State University
Las Cruces, NM

Director’s Postdoctoral Fellow, Los Alamos National Laboratory, 2006-2009
Ph.D. Chemical Engineering, Texas A&M University, College Station, TX 2005 
M.S. Chemical Engineering Texas A&M University, College Station, TX 2002
B.S. Chemical Engineering, New Mexico State University, Las Cruces, NM 2000
Scientific Interest and Avenues for Collaboration
Flow cytometry instrumentation development; fluorescence lifetime technologies; high-throughput fluorescence decay kinetic measurements; phase-sensitive flow cytometry; fluid mechanics
Participation / Support of ISAC
ISAC Councilor 2014-2018
Chair, Scientific Communications Committee (2014-present)
Member, ISAC Marylou Ingram Scholars Program Committee (2016-present)
Member, CYTO Organizing Committee (2014-2017)
Member, Awards Committee (2016-present)
Member, Nominating Committee (2016-present)
CYTO workshop evaluator, presenter, poster judge, session chair (2012-present)
Reviewer and protégé for Associate Editor of Cytometry Part A (2015-present)

Xu Huang

Lecturer/Principal Investigator of Haemato-Oncology/Systems Medicine Group
Paul O’Gorman Leukaemia Research Centre
Institute of Cancer Sciences, MVLS
University of Glasgow

2004 Ph.D., University of Peking, Beijing, P.R.China
2004-2008 Postdoctoral Researcher at MRC-Protein Phosphrylation Unit with Prof. Dario Alessi, Dundee, U.K.
2008-2014 Senior Research Associate at CRUK Manchester Institute with Dr. Tim Somervaille, Manchester, U.K.
Scientific Interest and Avenues for Collaboration
My lab has recently identified and validated few potential candidates as therapeutic treatment targets in Acute Myeloid Leukaemia (AML). (Huang et al. Leukaemia 2014, Harris and Huang et al. Cancer Cell 2013). The future research will focus on epigenetic regulation in leukaemia cancer stem cells, and continue to identify novel pathways and targets in leukaemia by using combinations of different systems biology approaches and by exploring novel polyomics methods in the study of cancer stem cells.
Participation/Support of ISAC
ISAC Scholar, 2014 – 2018
Attended CYTO regularly since 2014
Member of program committee for CYTO since 2014
Oral and Poster presentation reviewer and judge since 2015
Co-session chair at CYTO2015/2017

Hervé Luche

R&D Manager
Center for Immunophenomics (CIPHE)

Ph.D. in Immunology, Medical School of Ulm, Germany
Post-doctoral fellow on T cell and dendritic cell development in the laboratory
of B&M Malissen, France
Scientist at CIPHE, US-012, INSERM
Scientific Interest and Avenues for Collaboration
I am interested in trying to understand how immune cells integrate signals and
communicate with their environment to actually change their transcriptional programs, fate or acquire a new function. I use a combination of mouse genetics with high content multi-parameter flow and mass cytometry as well as transcriptomic analysis. I am looking for collaborations in the field of meta-analysis and data integration of multiple datasets.
Participation/Support of ISAC
Attended CYTO 2012, 2014, 2015, 2016, 2017
Member of Membership Services Committee (2016 – present)
Member of the CYTO 2016 Parallel Session Committee
Parallel session chair (2015-2016), workshop evaluator (2016), and poster judge (2014)
Volunteered for French translation of CYTO-U tutorial material
Promoting cytometry certification efforts in France

Yolanda Mahnke

Flow Cytometry Consultant
BSc in Biology; Imperial College of Science, Technology and Medicine, University of London, London, UK
PhD in Immunology with Prof. V Schirrmacher; The Open University, London, UK; and Dept. of Cellular Tumour Immunology, German Cancer Research Center, Heidelberg, Germany
PostDoc with Prof. V Schirrmacher, Dept. of Cellular Tumour Immunology, German Cancer Research Center, Heidelberg, Germany
PostDoc with Prof. P Romero, Div. of Clinical Onco-Immunology, Ludwig Institute for Cancer Research, Lausanne Branch, Lausanne, Switzerland
PostDoc with M Roederer, ImmunoTechnology Section, Vaccine Research Centre, National Institutes of Allergies and Infectious Diseases, National Institutes of Health, Bethesda, MD, USA
Senior Research Investigator, Translational and Correlative Studies Laboratory, University of Pennsylvania, Philadelphia, PA, USA
Scientific Interest and Avenues for Collaboration
I have a long-standing interest in multi-parameter flow cytometry, and used this tool to study the evolution of T-cell responses as well as other immune or tumour cell types in the context of tumours, infections, vaccinations, or cellular therapies. I now assist and train others in building multi-colour reagent panels, as well as in designing experiments, troubleshooting, data analysis and interpretation.
Participation/Support of ISAC
CYTO meeting attendance 2011, 2014, 2015, 2016
Developed a novel publication platform in Cytometry A for the publication of optimized multicolor immunofluorescence panels (OMIP), together with Mario Roederer and Pratip Chattopadhyay
Ad hoc reviewer for Cytometry A
Taught at pre-CYTO Workshop 2011
Lectured at CYTO 2011
Judged posters at CYTO 2014, 2015, 2016
Chairing a parallel session at CYTO 2015, 2016, 2017
Workshop evaluation CYTO 2016
Programming Committee Member CYTO 2017
ISAC Associated Societies Committee Member since 2016
ISAC Communications Committee Member since 2016

Carolina Wählby

Professor of Quantitative Microscopy
Centre for Image Analysis
Dept. of Information Technology and Science for Life Laboratory
Uppsala University

MSc in Molecular Biotechnology, Uppsala University, 1998, PhD in Digital Image Analysis with thesis on Algorithms for Applied Digital Image Cytometry at the Dept. of Information Technology, Uppsala University, 2003.
PostDoc at the Dept. of Genetics and Pathology, Uppsala University
Associate Professor in Digital Image Processing 2009.
Principal Investigator, Imaging Platform of the Broad Institute of Harvard and MIT.
Scientific Interest and Avenues for Collaboration
My research is focused on development of algorithms for image analysis of microscopy data with applications in biomedical research. This includes contributing to Broad’s free and open-source CellProfiler project, and development of advanced methods to quantify and mine information in microscopy images, primarily from high-throughput experiments and tissue samples. I’m interested in collaborations in the exciting intersection between computer science and biomedicine as microscopy is becoming a quantitative measurement tool, and not only a means of visual observation. I now lead a research group at Uppsala University, with support from SciLifeLab Sweden, the Swedish research council, and the ERC.
Participation/Support of ISAC
I’m interested in having a more active part in the ISAC community by attending meetings and networking activities.  I was a member of the CYTO 2016 Program Committee.

Emeritus Scholars

Term First Name Last Name  Organization  Country
2013-2017 Michael Halter NIST USA
  Er Liu Clearlight Diagnostics USA
  Yiqing Lu Macquarie University Australia
  Frank Schildberg University Hospital, Bonn Germany
  Joseph Tario, Jr. Roswell Park Cancer Institute USA
2012-2016 Nima Aghaeepour BC Cancer Agency Canada
  Pratip Chattopadhyay              New York University USA
  Sung Hwan Cho NanoSort, Inc. USA
  Anis Larby Singapore Immunology Network Singapore
  Enrico Lugli National Institutes of Health USA
  Bruno Paredes Institute of Biophysics Carlos Chagas Filho    Brazil
  Katarzyna Piwocka Nencki Institute of Experimental Biology    Poland
  Gergely Toldi Semmelweis University                Hungary
  Qianjun Zhang Emerald Biotech USA
  Michael Zordan iCyt Corporation USA
2011-2015 Donat Alpar Institute of Cancer Research-London    UK
  Kewal Asosingh Cleveland Clinic USA
  Alfonso Blanco-Fernandez University College Dublin Ireland
  Brian Grimberg Case Western Reserve University USA
  Tomáš Kalina Charles University, Prague        Czech Rep.
2012-2014 Rachael Walker Babraham Institute UK
2008-2012 Laura Adang University of Virginia            USA
  Zaha Almakhlafi King Faisal Specialist Hospital        Saudi Arabia
  Tytus Bernas University of Silesia Poland
  Estelle Glory Carnegie Mellon University USA
  Andrea Holme National University Medical Institute    Singapore
  Dayong Jin Macquarie University CLA Physics    Australia
  Silas Leavesley University of South Alabama        USA
2007-2011 Alireza Ardjmand Ghahestani    Tehran University of Medical Science    Iran
  Zoe Cohen University of Arizona USA
  Ji Fan Univeristy of Iowa USA
  Sach Jayasinghe Prince Henry's Institute        Australia
  Michael Lewis University of Vermont            USA
2006-2010 Ryan Brinkman Terry Fox Lab Canada
  Dario Coletti University of Rome la Sapienza Italy
  Dominik Lenz University of Freiburg Germany
  Claudio Panzarella                       ENEA Research Center, Casaccia Italy
  Reiner Schulte German Primate Center Germany
  Loretta             Yang                    National University of Ireland, Galway Ireland


Our vision is to advance the impact of cytometry in meeting current and emerging challenges in the life, biomedical, and physical sciences.

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