This website uses cookies to store information on your computer. Some of these cookies are used for visitor analysis, others are essential to making our site function properly and improve the user experience. By using this site, you consent to the placement of these cookies. Click Accept to consent and dismiss this message or Deny to leave this website. Read our Privacy Statement for more.
News & Press: ISAC News

A Major Step Towards Functional Single Cell Analysis in Cytometry Part A

Wednesday, June 20, 2018   (0 Comments)
Posted by: Casey Reiland
The May issue of Cytometry Part A: Journal of Quantitative Cell Science is now available!

In this May issue of Cytometry Part A read the editorial by our Editor-in-Chief Attila Tárnok, and learn about a major step towards functional single cell analysis.

A guide to choosing fluorescent protein combinations for flow cytometric analysis based on spectral overlap.

In this month’s Editor’s Choice article, Kleeman et al. guide researchers deciding on which fluorescent protein to use when creating a new reporter mouse or when testing fluorescent proteins that are being developed. The authors examine the performance of each fluorescent protein based on criteria such as brightness, degree of fluorescence spillover, and spreading error and demonstrate the utility of five fluorescent proteins with optimal characteristics for multicolor experiments, three of which can be easily used simultaneously.
Relative quantification of beta‐adrenergic receptor in peripheral blood cells using flow cytometry

Quantification of β‐AR expression has the potential to become an essential tool in clinical practice to evaluate response to treatment, to monitor disease activity and to make disease prognostics as well as understanding molecular basis of β‐AR dysregulation. Here, Saygin et al. describe an optimized method that will allow for large‐scale flow cytometric quantification of relative β‐AR expression on peripheral blood cells.

Monitoring circulating prostate cancer cells by in vivo flow cytometry assesses androgen deprivation therapy on metastasis

There is still controversy whether total androgen suppression by surgical castration prolongs survival patients with progressive prostate cancer. In this issue, Pang et al. use in vivo flow cytometry to monitor metastasis in tumor mouse models and to assess the curative effects of androgen deprivation therapy by surgical castration.

Read all articles included in this issue here.