From January 23rd to 24th, 2020, the 3rd German Mass Cytometry User Forum took place in Berlin. More than 100 international and national participants followed the extensive program for two days, organized by Henrik Mei, chair of the steering committee of the German Mass Cytometry Network (GerMaNet) and Scientific Head of the Mass Cytometry platform at the German Rheumatism Research Center, DRFZ, a Leibniz Institute.
The meeting intended to link mass cytometry laboratories in Berlin, Munich, Ulm, Cologne, Freiburg and Dresden, among each other and internationally to promote networking and exchange within the mass cytometry community in and around Germany.
With more than 100 visitors – mostly experts and users, but also novices to mass cytometry - from Germany and eleven other countries, the German Mass Cytometry User Forum has established itself as the leading hub for mass cytometry topics in Germany with international impact.
Seven invited speakers, two workshops and seven selected abstracts covered mass cytometry technology & reagents, different areas of biomedical research, experimental setup and controls, and computational data mining and visualization.
We received 24 abstract submissions which were displayed and discussed during the poster tour. Tomer Meir Salame, PhD (Flow Cytometry Unit, Life Sciences Core Facilities, Weizmann Institute of Science, Rehovot, Israel) won the poster award for his contribution on “High-Dimensional Single-Cell Mass Cytometry Analysis in Murine Models of Alzheimer´s Disease & Tauopathy upon PD-L1 immune checkpoint blockade”.
The International Society for Advanced Cytometry kindly supported the ISAC lecture given by Michael D. Leipold, PhD (Stanford University, School of Medicine) in which he shared his rich, likely unmatched experience with conducting mass cytometry studies, in particular large immune profiling studies. Dr. Leipold first covers data and lessons learned from a large cohort of samples run in a short period of time and compares procedures with a cohort of similar size that was acquired and run over the course of several years. He very much emphasized to be aware that large sample sizes require meticulous and thoughtful preparation from choosing study power, sample size, CyTOF instrument setup, barcoding, data analysis axes, to data storage and file transfer, all of which impact on how to conduct such studies optimally.
We deeply thank the International Society of Advancement of Cytometry (ISAC) for supporting Michael Leipold´s lecture during this exciting and fruitful conference. At the meeting, Dr. Leipold was introduced by Dr. Henrik Mei, who used the opportunity to introduce ISAC and its aims, and talk about CYTO 2021 and the Marylou Ingram Scholar program.