While ISAC's live cytometry courses and workshops have been extremely successful, participant access is limited by geography, time constraints, and finances, leaving much unmet demand. CYTO U aims to address this need by providing peer-reviewed courses, recorded courses and tutorials from CYTO conferences, and a topical webinar series, among other interactive elements. To view a webinar, register on ISAC's membership page and become either a dues-paying member or a free nonmember. CYTO U is designed to enable cytometrists from around the world to interact with leaders in the field and with each other, a mission that continues to expand the ISAC community and advance the careers of our users.
May 28, 2020 - Discovering Immune Features During COVID-19 By Advanced Cytometry
Presenter: Andrea Cossarizza, University of Modena and Reggio Emilia, ISAC President
Summary: The immune system is heavily involved in the pathogenesis of Covid-19, and its activation, which includes the so-called cytokine storm, and is the main force that drives the course of the infection. This webinar will present the most recent data on main changes that occur among different lymphocyte populations along with functional analysis of T cells, and discuss current and possible therapeutic approaches. Reserve Your Spot!>
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January 8, 2020 - Intelligent Image-Activated Cell Sorting: A Tutorial
Presenter: Keisuke Goda, University of Tokyo, University of California, Los Angeles, Wuhan University
Summary: The advent of intelligent Image-Activated Cell Sorting (iIACS) has enabled high-throughput intelligent image-based sorting of single live cells or cell clusters with unique morphochemical features that are difficult to discern when compressing these spatial data into intensity signals in fluorescence-activated cell sorting (FACS) [Nitta et al., Cell 175, 266-276 (2018)]. iIACS is an on-chip microfluidic technology that builds on a seamless integration of a high-throughput fluorescence microscope, cell focuser, cell sorter, and deep neural network on a hybrid software-hardware data management architecture, thereby providing the combined merits of optical microscopy, FACS, and deep learning [Isozaki et al., Nature Protocols 14, 2370-2425 (2019)]. Therefore, iIACS serves as an essential part of holistic single-cell analysis by providing direct connections between population-level analysis (flow cytometry), cell-level analysis (microscopy), and gene-level analysis (sequencing) of sorted cells. In this webinar, I will give a tutorial about the principles and applications of iIACS and compare the usability of iIACS with other technologies such as FACS, imaging flow cytometry (without sorting), and image-based cell pickers.
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December 18, 2019 - High-Speed Cell Sorter Biosafety and Containment Testing
Presenter: Geoffrey Lyon M.P.H. Yale Flow Cytometry Facility Manager/BSL-3 Sort Operator
Summary: This webinar explores the various biosafety aspects of high-speed cell sorting. The emphasis of this presentation is the evolution and development of aerosol containment testing, including the new ISAC standard that uses a novel impactor and microspheres to test containment. This new assay can be utilized to test sorters housed inside or outside of a biosafety cabinet.
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