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Minimum Information about a Flow Cytometry Experiment

A fundamental tenet of scientific research is that the published results of any study have to be open to independent validation or refutation. The Minimum Information about a Flow Cytometry Experiment (MIFlowCyt) establishes criteria for recording and reporting information about the flow cytometry experiment overview, samples, instrumentation and data analysis. It promotes consistent annotation of clinical, biological and technical issues surrounding a flow cytometry experiment by specifying the requirements for data content and by providing a structured framework for capturing information.

MIFlowCyt is:

  • An ISAC Recommendation
  • Endorsed by the Data Interoperability Steering Committee of the Division of Allergy, Immunology, and Transplantation within the National Institute of Allergy and Infectious Diseases (NIAID)
  • Supported by several scientific journals
  • Included in Minimum Information for Biological and Biomedical Investigations (MIBBI)

The latest MIFlowCyt specification is available here.

To learn more about the MFlowCyt standard, visit: