I started in flow cytometry in 1983 at the Ruby Lynch Cell Sorter Lab, Peter MacCallum Cancer Institute in Melbourne. At that time the lab was used for clinical and research work, including the diagnosis of AIDS patients at the beginning
of the HIV epidemic and early research on the isolation of haemopoietic stem cells. While working here I completed my Applied Physics degree at Royal Melbourne Institute of Technology.
In 1986, I joined the Department of Nephrology in Prince Henry’s Hospital to operate their cell sorter. During my time here I completed a Master of Science degree (Monash University) on macrophage function. In 1992, I joined the Clinical
Immunology department at Monash Medical Centre and was in charge of the core flow cytometry facility which was used for both clinical tests and research. I concurrently completed my PhD (Monash University) on the use of flow cytometry to quantify
immune function in kidney transplant recipients.
After working 24 years in Melbourne, in February 2008 I was appointed as the head of the Unidade de Citometria de Fluxo at the Instituo de Medicina Molecular in Lisbon, Portugal. This flow cytometry lab serviced more than 150 researchers,
and had a cell sorter, and three analysers. Since July 2009, I have been in charge of the core flow cytometry facility at the Life Sciences Institute of the National University of Singapore which has 4 analysers and 3 cell sorters.
I joined ISAC in 1986 and have attended many CYTO meetings since then. During my working stint in Australia, I was involved in the Australian Flow Cytometry Group (now known as the Australasian Cytometry Society) and was a key organising
committee member for the 2001 and 2007 annual meetings. My active involvement in conferences continued in helping to initiate and organise the first CYTOAsia conference in Singapore in 2017, which was a tremendous success with positive responses
from the region.
I am currently a member of the ISAC Live Education Task Force and have organised and participated in flow cytometry workshops in Singapore, Indonesia, India, Malaysia, and Vietnam since 2017. Besides running my SRF, I am also pursuing
research interests in using flow cytometry to develop diagnostic tests for tuberculosis and investigating new technologies for making single cell measurements.