Tomáš Kalina is currently an Associated Professor at Department of Pediatric Hematology and Oncology, Charles University in Prague, 2nd Faculty of Medicine, Czech Republic. He graduated MD in 2000 from 2nd Medical School, Charles University Prague and started his research and diagnostic carrier in Prague (leukemia diagnostics and biology) and continued onto a postdoctoral fellowship at Fred Hutchinson Cancer Research Center, Seattle, WA, USA (immune reconstitution post-BMT). He received a PhD from Immunology in 2005. He received the ISAC Scholar Award in 2010. He is a president of Czech Society for Analytical Cytometry, council member of International Society for Advancement of Cytometry, and a member of HCDM council. Throughout his career, he focused on development and translational application of flow cytometry-based techniques (immunodeficiency, immune monitoring, leukemia phenotyping, minimal residual disease monitoring, bead-based proteomics, and algorithmic data analysis). He is a founding member of EuroFlow consortium, where he was responsible for coordination of the technical aspects and design of flow cytometry procedures. At present, he is actively involved in primary immunodeficiency research, and he leads ISAC Live Education courses and EuroFlow proficiency testing. He develops new techniques and applications for polychromatic, spectral, and mass cytometry.