ISAC Biosafety Standards

The ISAC Biosafety Committee was established with the goal of providing guidance to its membership and the scientific community on safety issues related to cytometry, in particular cell sorting. The committee has been active in the creation of biosafety guidelines and standards for cell sorting and updated the Standards in 2014.

CLICK HERE for a Special Message from the ISAC Biosafety Committee Regarding COVID-19.

• 2014 ISAC Cell Sorter Biosafety Standards
  
• Revision to ISAC Cell Sorter Biosafety.pdf
  
• Webinar: High-Speed Cell Sorter Biosafety and Containment Testing
• Webinar: Laboratory Safety in a Flow Cytometry SRL

General Biosafety

Regulations and guidelines for risk assessment are available from a variety of government sources:

• WHO Lab Biosafety Guidance Related to Novel Coronavirus
• Biosafety in Microbiological and Biological Laboratories, 5th Ed. USA, NIH/CDC
• OSHA Bloodborne pathogens USA, “Universal Precautions” for handling samples containing potentially infectious material.
• Pathogen Safety Database: Public Health Agency of Canada Listing of Pathogens with risk assessment criteria.
• World Health Organization Laboratory Biosafety Manual: third edition of WHO biosafety manual in several languages.
• perfetto-2018-novel_impactor.pdf: Cytometry Part A article on the measurement of containment of aerosols generated in a flow cytometry cell

Cell Sorting Facilities: Standard Operating Procedures

Updated March 2020

• FCC Cell Sorting Facility SOPs
• FCC Cell Sorting Facility SOPs (Appendix)
• Infectious Cell Sorting Aerosol Containment Documentation
• BSL-2/3 (e.g. mycobacterium tuberculosis) Checklist Procedure
• Summary of the ARIA Operational Manual (Supplemental)