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Cytometry Part A
CYTOMETRY PART A - The Official journal of isac

In This Issue

June 2020 | Volume 97 | Issue 6

A Career in Cytometry R&D

by Robert A. (Bob) Hoffman

My career in cytometry has so far had four phases: as a post doc from 1976 to 1978 in the pioneering flow cytometry group at Los Alamos Scientific Laboratory (LANL); a scientist from 1978 to 1987 with Ortho Instruments (later expanded to Ortho Diagnostic Systems) an early flow cytometer manufacturer; as a scientist with Becton Dickinson (BD) from 1987 to 2009; and as a retiree still engaged in cytometry since 2009. I have been fortunate that both companies I worked for encouraged scientific engagement and collaboration with the cytometry community and also encouraged publication. . .

Aiming to Compare Apples to Apples: Analysis of Extracellular Vesicles and Other Nanosized Particles by Flow Cytometry

by André Görgens & John P. Nolan

Extracellular vesicles (EVs) are submicrometer‐sized biological vesicles released by all cells, and can be found in all body fluids or harvested from cell culture supernatants. It is nowadays widely accepted that EVs can serve as vesicular messengers in various physiological and pathophysiological contexts. Over the last 10–15 years, the EV research community has grown almost exponentially and the field has attracted a lot of attention following numerous studies connecting EVs to therapeutic approaches such as vaccination, antitumor therapy, immunomodulation and drug delivery. However. . .

Fluorescence and Light Scatter Calibration Allow Comparisons of Small Particle Data in Standard Units across Different Flow Cytometry Platforms and Detector Settings

by Joshua A. Welsh, Jennifer C. Jones, & Vera A. Tang

Flow cytometers have been utilized for the analysis of submicron‐sized particles since the late 1970s. Initially, virus analyses preceded extracellular vesicle (EV), which began in the 1990s. Despite decades of documented use, the lack of standardization in data reporting has resulted in a growing body of literature that cannot be easily interpreted, validated, or reproduced. This has made it difficult for objective assessments of both assays and instruments, in‐turn leading to significant hindrances in scientific progress, specifically in the study of EVs, where the. . .

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There was long time ago a saying by someone whose name I cannot recall at the moment: “Trust is good but control is better” (Or in other words: Доверие это хорошо. Контроль лучше). This is particularly true for quantitative science and I have...

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