by David Entenberg, Anja E. Hauser, & Raluca A. Niesner
Of the large variety of technologies that can be used to study mammalian physiology and pathophysiology, confocal and two‐photon intravital microscopy (IVM) is unique. For the past three decades, it has enabled researchers to monitor biological processes, live and in their true environment, all at high temporal and spatial resolutions. It is the only technology that provides single‐cell (and even subcellular) information, while maintaining the complex environment that exists only in living organisms. . .
by Juliane Handschuh, Jonas Amore, & Andreas J. Müller
During infections, interactions between host immune cells and the pathogen occur in distinct anatomical locations and along defined time scales. This can best be assessed in the physiological context of an infection in the living tissue. Consequently, intravital imaging has enabled us to dissect the critical phases and events throughout an infection in real time in living tissues. Specifically, advances in visualizing specific cell types and individual pathogens permitted tracking the early events of tissue invasion . . .
Monitoring of cell metabolism represents an important application area for fluorescence lifetime imaging microscopy (FLIM). In particular, assessment of mitochondrial membrane potential (MMP) in complex three‐dimensional multicellular in vitro, ex vivo, and in vivo models would enable improved segmentation and functional discrimination of cell types, directly report on the mitochondrial function and complement the quenched‐phosphorescence detection of cellular O2 and two‐photon excited FLIM of endogenous NAD(P)H. Here, we report the . . .
There was long time ago a saying by someone whose name I cannot recall at the moment: “Trust is good but control is better” (Or in other words: Доверие это хорошо. Контроль лучше). This is particularly true for quantitative
science and I have...