|What is Cytometry?|
what is cytometry?
The quantitative analysis of cells and cell systems.
Cytometry involves a wide range of cutting-edge techniques, most of which measure the molecular properties of cells by employing fluorescent labeling to detect specific antigens using antibodies, intracellular ions using indicator dyes, fluorescent reporter molecules such as green fluorescent protein (GFP), and DNA and RNA using nucleic acid-specific probes.
Flow cytometry is generally used for analyzing individual cells in a suspension, and is common in immunology and haematopathology. Hallmarks of flow cytometry are analysis speed, detection sensitivity, the ability to measure many parameters simultaneously, and the ability to sort individual cells.
Image cytometry is generally used for the multiparameter analysis of individual adherent cells and is used to measure many of the same parameters as flow cytometry. Image cytometry, however, carries the added ability of three-dimensional imaging. "High content analysis" is a term often applied to image cytometry in the context of high throughput screening, while "tissue cytometry" refers to the application of these techniques to cells in situ. Image cytometry is generally performed using automated microscopy and computational image processing and analysis.